[Federal Register: November 21, 1997 (Volume 62, Number 225)]

[Rules and Regulations]               

[Page 62243-62260]

From the Federal Register Online via GPO Access [wais.access.gpo.gov]

[DOCID:fr21no97-5]



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DEPARTMENT OF HEALTH AND HUMAN SERVICES



Food and Drug Administration



21 CFR Parts 809 and 864



[Docket No. 96N-0082]

RIN 0910-ZA03



 

Medical Devices; Classification/Reclassification; Restricted 

Devices; Analyte Specific Reagents



AGENCY: Food and Drug Administration, HHS.



ACTION: Final rule.



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SUMMARY: The Food and Drug Administration (FDA) is issuing a final rule 

to classify/reclassify analyte specific reagents (ASR's) presenting a 

low risk to public health into class I (general controls), and to 

exempt these class I devices from the premarket notification (510(k)) 

requirements. FDA is classifying/reclassifying ASR's used in certain 

blood banking tests as class II (special controls) because general 

controls are insufficient to provide a reasonable assurance of safety 

and effectiveness. Finally, ASR's presenting a high risk are being 

classified or retained in class III (premarket approval). FDA is also 

designating all ASR's as restricted devices under the Federal Food, 

Drug, and Cosmetic Act (the act), and establishing restrictions on 

their sale, distribution and use. The scope of products covered by this 

final rule includes both pre-1976 devices, which have not been 

previously classified, as well as post-1976 devices, which are 

statutorily classified into class III. The intent of this final rule is 

to regulate these pre- and post-1976 devices in a consistent fashion. 

This rulemaking does not affect requirements for reagents that are 

subject to licensure under the Public Health Service Act (the PHS Act). 

This rulemaking also does not affect reagents sold to nonclinical 

settings, including those reagents sold as components to manufacturers 

of cleared or approved in vitro diagnostic tests.



DATES: This rule is effective November 23, 1998.

FOR FURTHER INFORMATION CONTACT: Steven I. Gutman, Center for Devices 

and Radiological Health (HFZ-440), Food and Drug Administration, 2098 

Gaither Rd., Rockville, MD 20850, 301-594-3084.

SUPPLEMENTARY INFORMATION: 



I. Background



    The the act (21 U.S.C. 201 et seq.), as amended by the Medical 

Device



[[Page 62244]]



Amendments of 1976 (Pub. L. 94-295) (the amendments) and the Safe 

Medical Devices Act of 1990 (Pub. L. 101-629), established a 

comprehensive system for the regulation of medical devices intended for 

human use. Section 513 of the act (21 U.S.C. 360c) established three 

categories (classes) of devices, depending on the degree of regulatory 

controls needed to protect the public health. The three categories of 

devices are as follows: Class I, general controls; class II, special 

controls; and class III, premarket approval.

    Devices that were in commercial distribution before May 28, 1976 

(the date of enactment of the amendments), are classified under section 

360c of the act after FDA has: (1) Received a recommendation from a 

classification panel, an FDA advisory committee, (2) published the 

panel's recommendation for comment, along with a proposed regulation 

classifying the device; and (3) published a final regulation 

classifying the device. A device that is first offered in commercial 

distribution after May 28, 1976, and is substantially equivalent to a 

device classified under this scheme, is also classified into the same 

class as the device to which it is substantially equivalent.

    A device that was not in commercial distribution prior to May 28, 

1976, and that is not substantially equivalent to a preamendments 

device, is classified by statute into class III without any FDA 

rulemaking proceedings. FDA determines whether new devices are 

substantially equivalent to previously offered devices by means of the 

premarket notification procedure in section 510(k) of the act (21 

U.S.C. 360(k)) and part 807 of the regulations (21 CFR part 807).

    FDA held a meeting of its Immunology Devices Panel (the Panel) on 

January 22, 1996, to seek expert advice and public input on determining 

the regulatory controls to be placed on commercially marketed ASR's. 

ASR's are reagents composed of chemicals or antibodies that may be 

thought of as the ``active ingredients'' of tests that are used to 

identify one specific disease or condition. ASR's are purchased by 

manufacturers who use them as components of tests that have been 

cleared or approved by FDA and also by clinical laboratories that use 

the ASR's to develop in-house tests used exclusively by that 

laboratory. These in-house developed tests (sometimes referred to as 

``home brew'' tests) include those that measure a wide variety of 

antibodies used in the diagnosis of infectious diseases, cancer, 

genetic, and various other conditions.

    The Panel recommended that most ASR's be classified into class I 

because the Panel believed that general controls are sufficient to 

provide reasonable assurance of the safety and effectiveness of these 

ASR's. The Panel's recommendation for classification was based on the 

applicability of the general controls usually associated with class I 

products (e.g., registration, listing, current good manufacturing 

practice (CGMP), and medical device reporting), as well as the 

inclusion of restrictions on distribution, use and labeling. The Panel 

determined that the primary risks to health presented by ASR's sold to 

clinical laboratories are that they may be manufactured with variable 

quality, or be inappropriately labeled, or be used by persons without 

adequate qualifications. The Panel was also concerned that 

practitioners ordering the in-house tests made from ASR's may be 

unaware that the clinical performance characteristics of these tests 

have not been independently reviewed by FDA. In addition, the Panel 

identified a subset of ASR's whose use posed unique risks to public 

health because of the substantial clinical impact of the information 

generated using these devices.

    After the Panel meeting, FDA published a proposed rule to regulate 

ASR's (61 FR 10484, March 14, 1996). FDA received 31 comments on the 

proposed rule from individuals, manufacturers, professional societies, 

and consumer and health associations. The majority of the comments 

support the regulations proposed by FDA. A summary of the comments and 

FDA's response to them is provided below:



II. The Final Rule



A. General Approach



    The final rule classifies or reclassifies the majority of ASR's as 

class I medical devices. The final rule also exempts these class I 

devices from the premarket notification requirements of section 510(k) 

of the act. A small number of ASR's are being classified in class II or 

III because the agency has determined that additional requirements are 

necessary for their safe and effective use. Under the authority of 

section 520(e) of the act (21 U.S.C. 360j(e)), the final rule restricts 

the sale, distribution or use of all ASR's subject to the rule. FDA has 

determined that these restrictions are necessary to provide a 

reasonable assurance of the safety and effectiveness of ASR's, 

commensurate with their potentiality for harmful effect or the 

collateral measures necessary to their use. The final rule restricts 

ordering the use of in-house developed tests using ASR's to physicians 

or other health care practitioners authorized by applicable state law 

to access such tests. The final rule also restricts the sale of ASR's 

to those clinical laboratories regulated under Clinical Laboratory 

Improvement Amendments of 1988 (CLIA) as qualified to perform high 

complexity testing. In order to clarify that the rule is intended to 

allow ASR's to be sold to State laboratories exempt from CLIA 

certification, the language of the regulation has been modified to 

refer to laboratories ``regulated'' under CLIA rather than 

``certified'' under CLIA as had been proposed. In addition, to clarify 

that ASR's may be sold to Department of Veterans Affairs (Veterans 

Affairs) laboratories not covered by CLIA, the regulation has been 

modified to include Veterans Affairs laboratories regulated under 

comparable laws; currently that law is Pub. L. 102-139. The rule 

requires those laboratories covered by the regulation to provide a 

disclaimer with the results obtained through use of in-house developed 

tests incorporating these ASR's. The rulemaking does not affect 

reagents sold to nonclinical settings, including those sold as 

components to manufacturers of approved or cleared in vitro diagnostic 

tests. The rulemaking does not affect requirements for reagents that 

are subject to licensure under the PHS Act.



B. Class II or III ASR's



    FDA has identified a small subset of ASR's that require class II 

special controls to provide a reasonable assurance of safety and 

effectiveness; these are ASR's used in blood banking tests classified 

as class II devices where the underlying tests have already been 

cleared for marketing under section 510(k) of the act.

    Class II blood banking tests fall into two categories. One category 

consists of blood banking tests required by FDA that screen for 

diseases with a low potential for transmission. The second category 

consists of certain blood banking tests used electively by blood banks 

to screen for diseases that are likely to be transmitted to subsets of 

blood unit recipients known to be at greater risk of infection. An 

example of the second category is cytomegalovirus serological reagents, 

which are used in tests that aid in the diagnosis of diseases caused by 

cytomegaloviruses. An example of the first category is treponema 

pallidum nontreponemal test reagents, which are used in tests that aid 

in the diagnosis of syphilis.

    Class II ASR's will be subject to special controls that consist of 

the following National Committee for



[[Page 62245]]



Clinical Laboratory Standards (NCCLS) documents: (1) ``Specifications 

for Immunological Testing for Infectious Disease; Approved Guideline'' 

(December 1994, NCCLS Document I/LA18-A) and (2) ``Assessment of the 

Clinical Accuracy of Laboratory Tests Using Receiver Operating 

Characteristic (ROC) Plots; Tentative Guideline'' (December 1993, NCCLS 

Document KGP10-T) and the following FDA guidance documents: (1) 

``Review Criteria for Assessment of In Vitro Diagnostic Devices for 

Direct Detection of Mycobacterium spp.'' (July 6, 1993) and its 

``Attachment 1'' (February 28, 1994); (2)`` Draft Review Criteria for 

Nucleic Acid Amplification-Based In Vitro Diagnostic Devices for Direct 

Detection of Infectious Microorganisms'' (June 14, 1993); and (3) the 

Center for Biological Evaluation and Research's ``Points to Consider in 

the Manufacture and Clinical Evaluation of In Vitro Tests to Detect 

Antibodies to the Human Immunodeficiency Virus, Type I'' (54 FR 48943, 

November 28, 1989). FDA believes these special controls are sufficient 

to ensure safe and effective use of these ASR's because these ASR's 

have previously been evaluated in tests classified as class II and 

cleared by FDA.

    Persons interested in obtaining the documents previously referenced 

should refer to section IV in this document on ``Access to Special 

Controls.''

    In addition to the small subset of ASR's discussed above that have 

been identified as class III, FDA also has identified another small 

subset of ASR's for which class III premarket approval is necessary to 

protect the public health. These class III ASR's are those whose use 

poses unique risks because of the substantial clinical and public 

health impact of the information generated by using these devices. This 

subset of ASR's are those incorporated in tests intended to diagnose 

those contagious diseases that are highly likely to be fatal and where 

accurate diagnosis offers an opportunity to mitigate the public health 

impact of the condition or those ASR's incorporated in class III tests 

intended to establish the safety of blood and blood products, including 

genetic tests intended to ensure the safety of the blood supply. 

Examples of class III ASR's include ASR's used in tests to diagnose 

human immunodeficiency virus/acquired immune deficiency syndrome (HIV/

AIDS) or tuberculosis.

    Under Sec. 864.4020(b) (21 CFR 864.4020(b)), those analyte specific 

reagents that meet the class II or III ASR definition will be reviewed 

as a component of a test or kit. Because of the serious health risks 

associated with diseases diagnosed by tests utilizing class II or III 

ASR's, FDA believes that meaningful safety and effectiveness 

determinations require a review of the performance of the entire test 

or kit, including directions for use and expected analytical or 

clinical performance. Accordingly, FDA will undertake premarket review 

of the performance of the ASR and the test of which it is a component 

to determine the substantial equivalence or safety and effectiveness of 

class II and III ASR's. As a result, it is expected that most class II 

and III ASR's will not be marketed as independent components, separate 

from the test. Where manufacturers of the approved test or kit intend 

to market these class II and III ASR's independently, without the other 

components of the test, the restrictions issued under section 520(e) of 

the act will continue to apply. Cleared or approved class II or III 

ASR's that are marketed independently of kits may be sold only to in 

vitro diagnostic (IVD) manufacturers, laboratories qualified to do high 

complexity testing under CLIA, or nonclinical laboratories for research 

or other uses. These independently marketed ASR's must be labeled in 

accordance with Sec. 809.10(e) (21 CFR 809.10(e)), which has been 

amended to include the following statement: ``Except as a component of 

the approved test (Name of approved test), analytical and performance 

characteristics are not established.''

    Although manufacturers of Class II or III ASR's marketed as 

independent components are prohibited from making statements regarding 

the analytical or clinical performance of the ASR, they may identify 

the approved test or kit. Because the clinical laboratory is 

accountable for the use of the independently marketed ASR and its 

performance as a part of a test, the disclaimer required by 

Sec. 809.30(e) (21 CFR 809.30(e) must be appended to the results of in-

house developed tests using class II or III ASR's just as it is 

required with reports of results using class I ASR's. The same 

statement, of course, would not be applicable or required when test 

results are generated using the test that was cleared or approved in 

conjunction with review of the class II or III ASR.



C. General Controls



    The final rule requires biological or chemical manufacturers and 

suppliers of ASR's to register with FDA and provide FDA with a list of 

the ASR's they supply to laboratories for use in developing in-house 

tests. The final rule also requires manufacturers and suppliers to 

conform to CGMP requirements (part 820 (21 CFR part 820)), as 

applicable. The final rule further requires manufacturers and suppliers 

to comply with medical device report (MDR) requirements (21 CFR part 

803) and report to FDA adverse events that may have been due to the 

ASR's. FDA believes that these general controls address the risk to the 

public health presented by ASR's that may be manufactured with variable 

quality.

    To reduce the burden on industry of complying with CGMP's, 

manufactures and suppliers have until November 23, 1998 to comply with 

part 820.



D. General Purpose Reagents



    FDA has amended the definition of general purpose reagents to 

complement and be consistent with the ASR definition by adding language 

clarifying the distinction between ASR's and general purpose reagents.



E. Genetics Testing



    FDA does not intend, at this time, to regulate ASR's used in 

genetic testing differently from other restricted class I medical 

devices that are exempt from premarket notification requirements. The 

ASR regulations are drafted to classify most ASR's used to develop in-

house tests as class I devices because FDA believes this degree of 

regulatory control is commensurate with the need to bring consistency 

to the manufacture of these devices and to assure their safety and 

effectiveness when used by health and scientific personnel trained in 

laboratory practices.

    FDA considered identifying a subset of ASR's that are used to 

develop tests intended for predictive genetic diagnosis as ASR's that 

pose unique risks to the public health because of the substantial 

clinical impact of the information generated using these devices. For 

the genetic tests currently in use, FDA is aware that both the genetic 

test and the ASR used in the genetic test are developed by the 

laboratory in-house. Because these ASR's are not being commercially 

marketed independently of the tests, they do not currently fall within 

the scope of this regulation. Nonetheless, FDA considered designating 

as class III devices those ASR's that would be marketed independently 

for use in tests intended for use in overtly healthy people to identify 

a genetic predisposition to a dementing disease, or to fatal or 

potentially fatal medical disorders (e.g., cancers or Alzheimer's 

disease), in situations where penetrance is poorly defined or variable 

and latency is 5 years or longer. However, after reviewing the comments 

and currently



[[Page 62246]]



available information, FDA has not yet identified criteria that would 

logically distinguish among genetic tests in order to determine which 

have the requisite impact to trigger more stringent controls. FDA has 

determined that the special issues related to genetic testing or 

predictive genetic testing do not warrant establishing a more stringent 

degree of regulatory control over ASR's used in these tests at this 

time. FDA believes that regulating most ASR's as restricted class I 

devices exempt from premarket notification establishes appropriate 

initial controls in the event more stringent requirements are later 

determined to be necessary for ASR's used in genetic tests.

    FDA is aware of the public concern and desire that the regulation 

of products used in genetic testing be done in a thoughtful and prudent 

manner. As stated previously, FDA intends, with this regulation, to 

establish appropriate initial controls for ASR's use in genetic tests 

and to review agency policies relating to many aspects of regulation of 

genetic testing after FDA has had an opportunity to evaluate 

anticipated final recommendations from National Institute of Health's 

(NIH's) Task Force on Genetics Testing and other interested parties. 

After this review, FDA may propose additional regulation of genetic 

tests.



F. Definition of an ASR



    Most comments found FDA's proposed definition for an ASR to be 

acceptable. However, FDA has decided to make minor changes to clarify 

the definition in response to some comments. FDA has amended 

Sec. 864.4020(a) to clarify that the regulation only applies to 

reagents intended for use in a diagnostic application. FDA also has 

added the term ``ligand'' to the categories of materials that are 

within the definition of ASR because ligands bind the reagents to the 

analytes. Finally, FDA has amended the definition to clarify that 

binding between ASR's and their analytes may be through physical or 

chemical means.



G. Disclaimer



    Under Sec. 809.30, FDA is requiring that a disclaimer be appended 

by the laboratory to the test report informing the ordering 

practitioner of the test results obtained from the test in which the 

ASR was used. The statement will say, ``This test was developed and its 

performance characteristics determined by [Laboratory Name]. It has not 

been cleared or approved by the U.S. Food and Drug Administration.'' 

FDA believes the disclaimer clarifies the regulatory status of the test 

in which the ASR has been used, is consistent with other in vitro 

diagnostic labeling, and addresses the concern raised by the Panel that 

practitioners ordering the tests made from class I exempt ASR's or from 

class II or III ASR's marketed independently of an approved test may be 

unaware that the clinical performance characteristics of those tests 

have not been independently reviewed by FDA. The statement would not be 

applicable or required when test results are generated using the test 

that is cleared or approved in conjunction with review of the class II 

or III ASR. It will be FDA's responsibility to enforce the disclaimer 

requirement.



H. Sale Restrictions



    The final rule does not regulate the sale of ASR's to nonclinical 

laboratories. FDA has amended Sec. 809.30(a)(3) to clarify that ASR's 

may be sold for nonclinical uses or uses not directly related to 

patient care to academic and other research laboratories as well as to 

other nonclinical laboratories. It is not the intent of the ASR 

regulations to prevent the continued sale of ASR's to research 

institutions that are using these devices for nondiagnostic testing.



I. Labeling Changes and Ordering Restrictions



    FDA has amended Sec. 809.10(e)(9) to clarify that labeling for 

class I exempt ASR's must include the statement, ``Analyte Specific 

Reagent. Analytical and performance characteristics are not 

established.'' For class II and III ASR's, FDA has amended 

Sec. 809.10(e)(9) to clarify that labeling must include the statement 

``Analyte Specific Reagent. Except as a component of the approved/

cleared test (Name of approved/cleared test), analytical and 

performance characteristics are not established.'' Such labeling is 

consistent with other IVD labeling and provides accurate information to 

users and purchasers of these products.

    FDA has added Sec. 809.10(f) to restrict ordering in-house 

developed tests using ASR's to physicians or other health care 

practitioners authorized by the law of the State in which the test is 

being offered. FDA believes that interpretation of results from in-

house developed tests that use ASR's requires the expertise of a health 

care practitioner authorized by the State to provide a reasonable 

assurance of the safe and effective use of commercially marketed ASR's. 

Because the performance characteristics of the individual tests have 

not been cleared or approved by FDA, consumer use of such tests without 

the benefit of the experience of a health care professional would 

significantly undermine safe and effective use of these ASR's.



III. Response to Comments



A. Comments Received in Response to FDA's Solicitation of Opinions on 

Specific Issues



1. Genetic Testing

(Comment 1)

    Several comments supported regulating ASR's used in genetic testing 

as class I exempt devices. Those comments asserted that:

    (a) Use of genetic test results are better addressed through 

regulations pertaining to confidentiality of results, discrimination 

based on genetic information, and the qualifications of genetic 

counselors and physicians, and through standards and guidelines 

established by professional organizations rather than through more 

stringent device controls.

    (b) CGMP requirements, labeling restrictions, as well as CLIA 

requirements for qualifying laboratories to perform high complexity 

testing adequately, address FDA concerns about the safety and 

effectiveness of ASR's used for such tests.

    (c) More stringent classifications of ASR's used in genetic tests 

may hamper the availability of genetic testing, which would adversely 

affect the development and practice of genetic medicine by adding 

substantially to the time and expense associated with test development.

    (d) Clinical laboratories have the responsibility and expertise to 

validate genetic tests, to establish standard operating procedures so 

that tests can be consistently replicated by technicians, and to 

generate in-house reference standards to test any new reagent lot for 

specificity.

    (e) ASR's should not be singled out for more stringent 

classification because ASR's are only one component of the clinical 

assay; properties of the general reagents used in the assay, such as 

ionic strength, pH and concentration, as well as conditions and 

procedures at the test site, are also critical for determining 

analytical specificity.

    (f) Genetic tests are not fundamentally different from other 

diagnostic technologies.

    (g) The proposed ASR category would allow flexibility for medical 

decision making but a system that attempts to distinguish among 

different genetic categories of testing, such as diagnostic, carrier, 

population screening, or prenatal diagnosis, would be unwieldy.

    (h) Many ASR's could be unintentionally overregulated if a higher



[[Page 62247]]



classification was established for this group of ASR's because a 

majority of ASR's could be used as ingredients in a genetic test, even 

if they were not sold for that use.

    Other comments supported different treatment for ASR's used in 

genetic tests:

    (a) One comment suggested that it was premature to regulate ASR's 

composed of human genetic products as class I until the molecular basis 

of human disease is better understood. Another comment suggested that 

ASR's should be regulated as class III medical devices if the practice 

of making in-house assays of genetic tests directly available to 

consumers becomes widespread or problematic.

    (b) Two comments recommended that ASR's used in genetic screening 

tests for predictive purposes in apparently healthy persons should be 

regulated more strictly than class I, for example, by requiring 

premarket notification.

    (c) One comment proposed that ASR's whose only labeled indications 

are in the area of genetic predisposition or in prognostic situations 

with long latency periods should be regulated as class II or III 

devices.

    (d) Two comments proposed regulating ASR's used in genetic testing 

as class II devices. One comment proposed special controls for these 

ASR's and no exemption from notification. The second comment would 

allow the sale of ASR's to laboratories without regard to certification 

by CLIA.

    (e) Because the clinical validity of ASR's may be difficult to 

establish, their sensitivity and predictive value may not be high, and 

the benefits they confer are not proven, one comment recommended that 

ASR's used in genetic screening tests for predictive purposes in 

apparently healthy persons should be available on an investigational 

basis only. Another comment said they should be available on an 

investigative basis until clinical validity is proven, and then they 

should be classified as class III devices. Two comments recommended 

that they should be regulated as class III devices.

    In general, FDA agrees with those comments that support regulating 

ASR's used in genetic tests as class I exempt. (See the discussion in 

section II.E. of this document.) The regulations were issued to apply 

to ASR's as a category of device, and most ASR's can be used in a 

variety of in-house developed tests. At this time, FDA does not believe 

there is a scientific basis to distinguish between tests based on the 

use of DNA and tests based on the use of other proteins or substances, 

or between tests based on the use of DNA and tests based on the use of 

other molecular diagnostic technologies. However, FDA recognizes that 

there are special issues related to genetic testing or predictive 

genetic testing and that these issues may affect the degree of 

regulatory control needed to establish the safety and effectiveness of 

these tests or the ASR's used in their development. As stated 

previously, FDA intends to review its decision with respect to 

regulatory control of genetic testing after it has had an opportunity 

to evaluate final recommendations from NIH's Task Force on Genetics 

Testing and other interested parties.

    FDA believes that this final regulation will assure the quality of 

material being used to develop in-house genetics tests. When used as 

part of in-house developed tests, the ASR regulations restrict use of 

commercially marketed ASR's to tests that are ordered by an authorized 

practitioner and to those clinical laboratories regulated under CLIA as 

qualified to perform high complexity testing. Except when test results 

are generated using the test that was cleared or approved in 

conjunction with review of the class II or III ASR, FDA is also 

requiring that a disclaimer be appended to the test report stating that 

the clinical laboratory determined and developed the test performance 

characteristics and that the test that incorporated the ASR has not 

been cleared or approved by FDA. FDA believes these restrictions 

address many of the concerns raised by those comments supporting more 

stringent regulation of ASR's used in genetic testing. The issuance of 

these regulations does not preclude FDA from reevaluating in the future 

whether additional controls may be needed for genetics testing or for 

ASR's used in such tests. FDA will reevaluate whether additional 

controls may be needed to provide an appropriate level of consumer 

protection if further developments in this area result in significant 

uses of ASR's in genetic assays or other IVD tests offered over-the-

counter (OTC).

(Comment 2)

    One comment stated that issues raised by predictive testing which 

yields information about the potential future health status of the 

patient and his or her blood relatives have been addressed by policy 

statements from professional groups. This comment asserted that the 

most practical approach to oversight and regulation of genetic testing 

would build on the existing system of professional society standards, 

using a system that creates either incentives for compliance or 

disincentives for noncompliance. The comment also stated that reliance 

on voluntary professional standards would minimize costs to Government 

agencies and avoid burdening compliant manufacturers with unnecessary 

regulation. Another comment recommended that regulation of human 

genetic testing should be considered separately from decisions 

regarding the appropriate classification and regulatory controls 

applied to ASR's.

    As stated previously, FDA recognizes that there are special issues 

related to genetic testing or predictive genetic testing. 

Implementation of a system based on professional standards for 

oversight of genetic testing is one option for addressing these issues. 

FDA does not believe the regulatory steps being taken in this final 

rule overly burden manufacturers or preclude other types of controls in 

the future, including systems based on the principles described in this 

comment.

2. Nucleic Acids

(Comment 3)

    Several comments agreed with FDA's proposal to include human 

nucleic acids within the definition of ASR's. Those comments stated 

that: (a) It would be inconsistent to exclude human nucleic acids; (b) 

human nucleic acids are essential for good patient management where no 

FDA approved alternative test can substitute; (c) the scientific basis 

for nucleic acid hybridization and amplification techniques utilizing 

oligonucleotide ASR's have been known for many years so that adherence 

to CLIA regulations should be sufficient regulation; (d) because 

factors affecting test performance, reliability, and accuracy of test 

results are assay dependent and not disease dependent, all ASR's should 

be regulated similarly as class I devices exempt from premarket 

notification; (e) the ongoing refinement of reagents for diagnosis of 

susceptibility genes required by the practice of medicine is 

facilitated when ASR's are required only to meet a minimum number of 

regulatory requirements; (f) the availability of nucleic acid probes 

for use in the practice of medicine will be facilitated if these 

nucleic acids are regulated as class I devices exempt from the 

premarket notification requirement; and (g) like other ASR's, human 

nucleic acids can be used in disease staging.

    Several comments supported the exclusion of the word ``nonhuman'' 

to modify nucleic acids in the ASR definition, stating that it would be 

virtually impossible to distinguish between a nucleic acid synthesized 

in the laboratory and a human nucleic acid, and that human nucleic 

acids are



[[Page 62248]]



not the only category of ASR capable of being used in genetic tests. 

One comment expressed concern that FDA has appeared to misunderstand 

the panel's intent, which was to exclude human nucleic acids because 

they are most often used to directly identify genetic material or gene 

products.

    FDA agrees with the comments that support including human nucleic 

acids in the ASR definition. FDA appreciates the basis for the concern 

raised by the comment about the intent of the panel recommendation, but 

remains concerned about the broad nature of such an exclusion. 

Consequently, the definition of ASR's in the final rule includes human 

nucleic acids. As discussed earlier, at a future date, FDA may 

reevaluate whether additional controls over genetic tests are 

appropriate.

3. Analyte Specific Reagent

(Comment 4)

    Several comments supported the use of the term ``analyte specific 

reagent'' and no comment suggested an alternative.

    Accordingly, FDA has retained this term in the final regulation.

4. Disclaimer

(Comment 5)

    Several comments agreed with the proposed disclaimer, noting that 

it clarifies the regulatory status of ASR's, it is consistent with the 

current practice of labeling research or investigational IVD's, and it 

provides an incentive for laboratories to have their assays approved or 

cleared.

    Several comments supported having a disclaimer, but would like it 

to contain more information, including that the clinical performance of 

the test has not been established, that neither the laboratory test nor 

the procedures used to obtain the results have been reviewed by FDA, 

and that the ASR manufacturer is accountable for the ASR.

    Other comments suggested that the disclaimer be deleted, or, at a 

minimum, amended to read that the laboratory assay used to report these 

results has been validated in accordance with the requirements of CLIA. 

One comment would amend the disclaimer to read as follows:

    The reagents used in this test are regulated by the Food and Drug 

Administration (FDA) under the general controls of the Food, Drug, and 

Cosmetic Act (FDC Act). The regulations that implement the FDC Act 

require compliance with current good manufacturing practices (CGMP), 

accurate labeling and adverse event reporting, among others. The 

distribution of these reagents is limited to manufacturers of in vitro 

tests, laboratories qualified to perform high complexity testing and 

forensic and underwriter laboratories. This test was validated in 

accordance with the provisions of the Clinical Laboratory Improvement 

Amendments (CLIA'88). The program is managed by another federal agency, 

the Health Care Financing Administration (HCFA). (Laboratory Name) was 

certified/recertified by HCFA on (date) as a high complexity laboratory 

that is in compliance with CLIA regulations.

    Three comments opposed requiring any disclaimer, claiming it has no 

impact on the final diagnosis and is an intrusion on the process of 

medical interpretation. One of these comments suggested that it would 

be more reasonable to require the laboratory director to provide 

interpretive reporting to the physician.

    FDA has considered the comments and has determined to require the 

disclaimer discussed in the proposed rulemaking. FDA believes that the 

disclaimer is sufficiently clear to communicate that the test that used 

the ASR was developed, and its performance characteristics defined, by 

the laboratory without FDA review. FDA believes this statement clearly 

communicates to health care providers the regulatory status of the in-

house test that has used the ASR. FDA believes this labeling 

requirement is necessary to address the concern raised by the Panel 

that physicians may not be aware that the results of the testing they 

order using ASR's are generated by tests that have not been 

independently reviewed by FDA. Rather than being an intrusion on 

medical interpretation, the required statement ensures that health care 

providers have additional information upon which to make independent 

judgments. This labeling requirement would not be applicable or 

required when test results are generated using the test that was 

cleared or approved in conjunction with review of the class II or III 

ASR. FDA does not believe a more detailed or lengthy statement is 

necessary.



B. General Comments



(Comment 6)

    Several comments supported the regulation of ASR's as class I 

devices, exempt from premarket notification requirements in section 

510(k) of the act. These comments stated that: (a) The CLIA regulations 

regarding in-house modification of materials or methods are adequate to 

protect the health and well-being of patients without increasing the 

regulatory burden on manufacturers and laboratories or overloading 

FDA's already encumbered review process by classifying ASR's in a more 

stringent category; (b) in-house modification of materials and methods 

falls within the scope of the practice of medicine, and a more 

stringent classification would hamper the ability to provide quality 

medical services and care to patients, such as diagnostic work 

performed by pathologists; (c) stringent regulation of in-house 

modified or developed materials and methods would constrain the 

development of new and better technologies and the improvement of 

existing IVD technologies; and (d) a substantial and appropriate 

measure of control is gained by the regulation announced in the 

proposed rule.

    As recommended in these comments, FDA is finalizing the class I 

exempt classification as the classification for most ASR's.



(Comment 7)

    One comment expressed concern that the proposed regulation would 

put companies that have made the investment to obtain clearance of 

510(k)'s for class II antibodies at a competitive disadvantage if 

antibodies that are currently classified as class II are reclassified 

as class I devices exempt from premarket notification.

    FDA disagrees with this comment. Manufacturers that have submitted 

or intend to submit antibodies for review as class II test systems 

would be allowed to market those devices with clear intended uses and 

indications for use, instructions for use, and appropriate definition 

of performance parameters. Manufacturers of class I exempt ASR's will 

be required to limit their labeling to a description of the identity 

and purity (including source and method of acquisition) of the ASR in 

addition to standard information already required for general purpose 

reagents (e.g., net weight; storage instructions). Sale of class I 

exempt ASR's is also restricted in accordance with other restrictions 

listed in 21 CFR 809.30(b), while manufacturers of class II test 

systems cleared by FDA would be allowed to market those devices without 

regard to the restrictions in 809.30.



(Comment 8)

    One comment questioned whether classification of class III ASR's by 

the type of test for which it is to be used will create a quagmire of 

regulations, resulting in numerous exceptions to the class I status, 

confusion about how ASR's that can be used in multiple tests will be 

regulated, and the difficulty of distinguishing one fatal illness, such 

as HIV/AIDS, from another, such as herpes encephalitis.

    FDA believes that through a narrow definition of the class II and 

III identification, the exceptions to the general ASR classification 

have been limited to a manageable number. Under the final rule, 

exceptions to the ASR class I exempt classification are analytes



[[Page 62249]]



used in developing a test intended for use in the: (a) Diagnosis of a 

contagious condition that is likely to result in a fatal outcome and 

where prompt accurate diagnosis offers the opportunity to mitigate the 

public health impact of the condition; (b) screening of a condition for 

which FDA has established a recommendation or requirement for the use 

of the test in safeguarding the blood supply or establishing the safe 

use of blood and blood products (e.g., hepatitis or tests for 

identifying blood groups); or (c) screening for blood banking when 

screening test has been classified as a class II device. Currently, FDA 

believes that ASR's used to test for evidence and monitoring for levels 

of HIV/AIDS and tuberculosis (TB) are examples that would fall within 

the class III exception, and reagents used in the diagnosis of diseases 

caused by cytomegaloviruses and treponema pallidum nontreponemal test 

reagents which aid in the diagnosis of syphilis fall within the class 

II exception.

    Most blood banking tests fall into class III and some into class 

II. Class II blood banking tests fall into two categories. One category 

consists of blood banking tests required by FDA to screen for diseases 

with a low potential for transmission, e.g., syphilis. The second 

category consists of certain blood banking tests used electively by 

blood banks to screen for diseases that are likely to be transmitted to 

subsets of blood unit recipients known to be at greater risk of 

infection, e.g., cytomegalovirus. Because these blood banking tests 

have previously been classified into class II, FDA has determined that 

special controls are sufficient and that the submission of a premarket 

approval application (PMA) associated with a class III device is not 

necessary for the ASR used in the test.



(Comment 9)

    One comment suggested that only those ASR's with the lowest risk 

factor for generating false results of little consequence should be 

classified as class I, and that the others should be classified as 

class II or III. The comment reasoned that the reliable, reproducible 

performance of a diagnostic test is dependent upon the entire 

integration of the test system. The comment also stated that while 

laboratories qualified to do high complexity testing have experience in 

utilizing and evaluating test systems developed by manufacturers, these 

laboratories do not have expertise in developing in vitro diagnostic 

tests. The comment noted that CLIA does not require the validation of 

diagnostic tests systems by rigorously controlled clinical trials to 

establish expected values and performance characteristics. Such trials 

are not required by CLIA but could be required by FDA if these tests 

were placed in class II or III.

    FDA has considered this and related comments and appreciates the 

concerns raised about the development of in-house tests and the current 

marketing of test services based on tests that have not been reviewed 

independently for safety and effectiveness. FDA believes that clinical 

laboratories that develop such tests are acting as manufacturers of 

medical devices and are subject to FDA jurisdiction under the act. 

However, FDA recognizes that the use of in-house developed tests has 

contributed to enhanced standards of medical care in many circumstances 

and that significant regulatory changes in this area could have 

negative effects on the public health. For these reasons, FDA declines 

to accept the suggestion that all in-house developed tests be 

classified as class II or III medical devices. FDA views this final 

rule as a reasonable regulatory step at this time and an important 

contribution to assuring that the primary ingredients of most in-house 

developed tests are manufactured properly, used by trained 

professionals, and labeled accurately.

    The focus of this rule is the classification and regulation of 

ASR's that move in commerce, not tests developed in-house by clinical 

laboratories or ASR's created in-house and used exclusively by that 

laboratory for testing services. The regulation restricts the sale of 

ASR's to a particular type of laboratory and FDA believes this 

restriction supports the safe and effective use of these ASR's. FDA 

believes that CLIA regulated laboratories qualified to perform high 

complexity testing have demonstrated expertise and ability to use ASR's 

in test procedures and analyses. In addition, the disclaimer being 

required by this rule will